Affordable Access

deepdyve-link
Publisher Website

Investigations on the use of fluorescence dyes for labeling dendrimers: cytotoxicity, accumulation kinetics, and intracellular distribution.

Authors
  • Scutaru, A M
  • Krüger, M
  • Wenzel, M
  • Richter, J
  • Gust, R
Type
Published Article
Journal
Bioconjugate Chemistry
Publisher
American Chemical Society
Publication Date
Dec 15, 2010
Volume
21
Issue
12
Pages
2222–2226
Identifiers
DOI: 10.1021/bc1001906
PMID: 21049938
Source
Medline
License
Unknown

Abstract

Fluorescent dyes (e.g., dansyl, fluoresceine isothiocyanate, or naphthalimide groups) are widely used as markers to study biological properties of drugs. In order to evaluate possible mediated cytotoxicity, we attached three molecules each to 1,3,5-tris(3-propylamino)benzene initially synthesized as core molecule for the design of dendrimers. Cytotoxic effects were only observed for the NO(2)-substituted naphthalimide conjugate. The intracellular distribution was visualized via confocal fluorescence microscopy and pointed to an accumulation in the endosome or nucleus, dependent on the cell line used.

Report this publication

Statistics

Seen <100 times