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Investigations on antipyrine metabolism. II--Analysis of conjugated metabolites of antipyrine in rat and human urine by off-line combination of liquid chromatography and field desorption mass spectrometry.

Authors
  • Lehmann, W D
  • Böttcher, J
  • Bässmann, H
  • Schüppel, R
  • Schiebel, H M
Type
Published Article
Journal
Biomedical mass spectrometry
Publication Date
Nov 01, 1982
Volume
9
Issue
11
Pages
477–482
Identifiers
PMID: 7159683
Source
Medline
License
Unknown

Abstract

The complex pattern of conjugates in the metabolism of antipyrine in man and rat has been investigated using an off-line combination of liquid chromatography and field desorption mass spectrometry. Using this approach, field desorption mass spectrometry enables the direct identification of underivatized glucuronides and sulphates in antipyrine metabolism on the basis of the corresponding protonated or cationized molecules representing the base peaks in the field desorption mass spectra. All conjugated metabolites of antipyrine established so far--four glucuronides and three sulphates--were identified in extracts of urine after stepwise purification by column chromatography and subsequent mass spectrometric analysis.

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