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Investigation on the validity of 3D micro-CT imaging in the fish brain.

Authors
  • Udagawa, Shingo1
  • Miyara, Keitaro2
  • Takekata, Hiroki3
  • Takeuchi, Yuki4
  • Takemura, Akihiro5
  • 1 Graduate School of Engineering and Science, University of the Ryukyus, 1 Senbaru, Nishihara, Okinawa 903-0213, Japan. , (Japan)
  • 2 Department of Chemistry, Biology and Marine Science, Faculty of Science, University of the Ryukyus, 1 Senbaru, Nishihara, Okinawa 903-0213, Japan. , (Japan)
  • 3 Organization for Research Promotion, University of the Ryukyus, 1 Senbaru, Nishihara, Okinawa 903-0213, Japan. , (Japan)
  • 4 Department of Chemistry, Biology and Marine Science, Faculty of Science, University of the Ryukyus, 1 Senbaru, Nishihara, Okinawa 903-0213, Japan; Okinawa Institute of Science and Technology Graduate University, 1919-1, Onna, Okinawa 904-0495, Japan. , (Japan)
  • 5 Department of Chemistry, Biology and Marine Science, Faculty of Science, University of the Ryukyus, 1 Senbaru, Nishihara, Okinawa 903-0213, Japan. Electronic address: [email protected] , (Japan)
Type
Published Article
Journal
Journal of neuroscience methods
Publication Date
Aug 28, 2019
Volume
328
Pages
108416–108416
Identifiers
DOI: 10.1016/j.jneumeth.2019.108416
PMID: 31472188
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Micro-computed tomography (CT) is a non-invasive technique that is used to obtain three-dimensional (3D) images of tissue structure in small animals. Compared with extensive mammal studies, few 3D imaging studies of fish have been conducted using micro-CT. An optimized method for imaging fish tissue structure is necessary, because they have adapted to diverse environments via functional and structural specialization. Brains of three species with different sizes and habitats were fixed in 4% paraformaldehyde and immersed in non-ionic iodinated contrast agent (Iopamiron). We examined the relationship between Iopamiron concentration and immersion time to determine universally optimal conditions for use in fish. We reconstructed 3D images of whole fish brains from cross-sections of brains from the Malabar grouper (Epinephelus malabaricus), bastard halibut (Paralichthys olivaceus), and threespot wrasse (Halichoeres trimaculatus). Developmental changes in brain structure were observed in the bastard halibut. Most brain regions of the threespot wrasse were distinguishable, although inner regions of the brain were less visible. Histological techniques are typically used to observe fish brain structure, despite its drawbacks in terms of tissue sample preparation (shrinkage and distortion) and image capture (3D image constriction). The technique examined in the present study solves these problems and allows for the simultaneous handling of multiple specimens. Micro-CT imaging is suitable for observing the surfaces and inner structures of fish of various species. Copyright © 2019 Elsevier B.V. All rights reserved.

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