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Investigation of relationships between barley stress peptides and beer gushing using SDS-PAGE and MS screening.

Authors
  • Hégrová, Blanka
  • Farková, Marta
  • Macuchová, Simona
  • Havel, Josef
  • Preisler, Jan
Type
Published Article
Journal
Journal of Separation Science
Publisher
Wiley (John Wiley & Sons)
Publication Date
Dec 01, 2009
Volume
32
Issue
23-24
Pages
4247–4253
Identifiers
DOI: 10.1002/jssc.200900510
PMID: 19950350
Source
Medline
License
Unknown

Abstract

The relationship between gushing and antifungal peptides in barley and malt kernels was examined for five barley varieties produced in the Czech Republic with four conditions of infection and treatment. Proteome changes during pathogen-seed interaction were observed with SDS-PAGE and MALDI-TOF MS. These methods were applied as a fast screening for observing the relationship between gushing and peptides/proteins. It was found that the presence of basic peptides, presumably hordothionins and non-specific lipid transfer protein type 1, did not correlate with the degree of gushing for malt (/r/ in <0.07, 0.34>), (/r/ in <0.01, 0.49>), respectively, as detected by both methods.

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