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Investigation of opioid receptor binding protein in adult and fetal mouse brain membranes by photoaffinity labeling.

Authors
  • Yeung, C W
  • Doyle, S
  • Ellard, S
Type
Published Article
Journal
Neuroscience Letters
Publisher
Elsevier
Publication Date
Jan 22, 1988
Volume
84
Issue
2
Pages
213–218
Identifiers
PMID: 2829063
Source
Medline
License
Unknown

Abstract

125I-[D-Ala2,p-N3-Phe4-Met5]Enkephalin was used to investigate the opioid receptor from brain membranes of adult (6 months old) and fetal (16 days gestation) mice. The photolabeled membranes were analyzed by sodium dodecyl sulfate gel electrophoresis. A 46,000-Da protein was specifically photo-labeled in the adult mouse brain membranes. The photolabeling of this protein was inhibited in the presence of [D-Ala2,Met5]enkephalin and a peptide ligand specific for the mu-opioid receptor. No specific labeling of any protein was detected in the brain membranes of the fetal mice. This apparent lack of the opioid receptor in the fetal mouse brain suggests that the appearance of the opioid receptor in the adult mouse brain is subject to developmental regulation.

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