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Investigation of ascorbate-mediated iron release from ferric phytosiderophores in the presence of nicotianamine.

Authors
  • Weber, Günther
  • von Wirén, Nicolaus
  • Hayen, Heiko
Type
Published Article
Journal
Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine
Publication Date
Oct 01, 2008
Volume
21
Issue
5
Pages
503–513
Identifiers
DOI: 10.1007/s10534-008-9137-8
PMID: 18322653
Source
Medline
License
Unknown

Abstract

Phytosiderophores (PS) are strong iron chelators, produced by graminaceous plants under iron deficiency. The ability of released PS to chelate iron(III), and subsequent uptake of this chelate into roots by YS1-type transport proteins, are well-known. The mechanism of iron release from the stable chelate inside the plant cell, however, is unclear. One possibility involves the reduction of ferric PS in the presence of an iron(II) chelator via ternary complex formation. Here, the conversion of ferric PS species by ascorbate in the presence of the intracellular ligand nicotianamine (NA) has been investigated at cytosolic pH (pH 7.3), leading to the formation of a ferrous NA chelate. This reaction takes place when supplying Fe(III) as a chelate with 2'-deoxymugineic acid (DMA), mugineic acid (MA), and 3-epi-hydroxymugineic acid (epi-HMA), with the reaction rate decreasing in this order. The progress of the conversion of ferric DMA to ferrous NA was monitored in real-time by high resolution mass spectrometry (FTICR-MS), and the results are complemented by electrochemical measurements (cyclic voltammetry), which allows detecting reactive intermediates and their change with time at high sensitivity. Hence, the combined results of electrochemistry and mass spectrometry indicate an ascorbate-mediated mechanism for the iron release from ferric PS, which highlights the role of ascorbate as a simple, but effective plant reductant.

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