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Investigation of antioxidative effects of a cardioprotective solution in heart tissue.

Authors
  • Russ, Miriam1
  • Jauk, Susanne1
  • Wintersteiger, Reinhold1
  • Andrä, Michaela2
  • Brcic, Iva3
  • Ortner, Astrid4
  • 1 Department of Pharmaceutical Chemistry, Institute of Pharmaceutical Sciences, University of Graz, Schubertstraße 1, 8010, Graz, Austria. , (Austria)
  • 2 Division of Transplant Surgery, Medical University of Graz, Auenbruggerplatz 29, 8036, Graz, Austria. , (Austria)
  • 3 Diagnostic & Research Institute of Pathology, Medical University of Graz, Neue Stiftingtalstraße 6, 8010, Graz, Austria. , (Austria)
  • 4 Department of Pharmaceutical Chemistry, Institute of Pharmaceutical Sciences, University of Graz, Schubertstraße 1, 8010, Graz, Austria. [email protected] , (Austria)
Type
Published Article
Journal
Molecular and Cellular Biochemistry
Publisher
Springer-Verlag
Publication Date
Nov 01, 2019
Volume
461
Issue
1-2
Pages
73–80
Identifiers
DOI: 10.1007/s11010-019-03591-y
PMID: 31342300
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

A multi-component solution, containing α-ketoglutaric acid (α-KG), 5-hydroxymethylfurfural (5-HMF), N-acetyl-seleno-L-methionine (NASeLM), and N-acetyl-L-methionine (NALM) as active ingredients, has been tested considering its supposed antioxidative effect with respect to heart transplantations. Oxidative stress was induced on isolated rat hearts through occlusion of a coronary artery and in chicken heart tissue through hydrogen peroxide. Both heart types were analyzed and the oxidative stress markers malondialdehyde (MDA) and carbonyl proteins (CPs) were determined via HPLC/UV-Vis. In both approaches, it was found that treatment with the multi-component solution led to a lower amount of MDA and CPs compared to a negative control treated with Krebs-Ringer solution (KRS). Further investigation on chicken heart tissue identified α-KG as antioxidative component in these experiments. However, numerous factors like arrhythmia, vessel dilatation, and minimization of oxidative stress effects play an important role for successful transplantation. Therefore, the investigated multi-component solution might be a novel approach against oxidative stress situations, for example at ischemia reperfusion injury during heart transplantations.

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