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An investigation of antioxidant and anti-inflammatory activities from blood components of Crocodile (Crocodylus siamensis).

Authors
  • Phosri, Santi
  • Mahakunakorn, Pramote
  • Lueangsakulthai, Jiraporn
  • Jangpromma, Nisachon
  • Swatsitang, Prasan
  • Daduang, Sakda
  • Dhiravisit, Apisak
  • Thammasirirak, Sompong
Type
Published Article
Journal
The protein journal
Publication Date
Oct 01, 2014
Volume
33
Issue
5
Pages
484–492
Identifiers
DOI: 10.1007/s10930-014-9581-y
PMID: 25216803
Source
Medline
License
Unknown

Abstract

Antioxidant and anti-inflammatory activities were found from Crocodylus siamensis (C. siamensis) blood. The 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) radical scavenging, nitric oxide scavenging, hydroxyl radical scavenging and linoleic peroxidation assays were used to investigate the antioxidant activities of the crocodile blood. Results show that crocodile blood components had antioxidant activity, especially hemoglobin (40.58 % nitric oxide radical inhibition), crude leukocyte extract (78 % linoleic peroxidation inhibition) and plasma (57.27 % hydroxyl radical inhibition). Additionally, the anti-inflammatory activity of the crocodile blood was studied using murine macrophage (RAW 264.7) as a model. The results show that hemoglobin, crude leukocyte extract and plasma were not toxic to RAW 264.7 cells. Also they showed anti-inflammatory activity by reduced nitric oxide (NO) and interleukin 6 (IL-6) productions from lipopolysaccharide (LPS)-stimulated cells. The NO inhibition percentages of hemoglobin, crude leukocyte extract and plasma were 31.9, 48.24 and 44.27 %, respectively. However, only crude leukocyte extract could inhibit IL-6 production. So, the results of this research directly indicate that hemoglobin, crude leukocyte extract and plasma of C. siamensis blood provide both antioxidant and anti-inflammatory activities, which could be used as a supplementary agent in pharmaceutical products.

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