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Investigating sesquiterpene biosynthesis in Ginkgo biloba: molecular cloning and functional characterization of (E,E)-farnesol and α-bisabolene synthases

Authors
  • Parveen, Iffat1
  • Wang, Mei1
  • Zhao, Jianping1
  • Chittiboyina, Amar G.1
  • Tabanca, Nurhayat1
  • Ali, Abbas1
  • Baerson, Scott R.2
  • Techen, Natascha1
  • Chappell, Joe3
  • Khan, Ikhlas A.1
  • Pan, Zhiqiang2
  • 1 University of Mississippi, National Center for Natural Products Research, Thad Cochran Research Center, School of Pharmacy, University, MS, 38677, USA , University (United States)
  • 2 United States Department of Agriculture, Agricultural Research Service, Natural Products Utilization Research Unit (USDA-ARS-NPURU), University, MS, 38677-1848, USA , University (United States)
  • 3 University of Kentucky, Department of Pharmaceutical Sciences, Lexington, KY, 405036, USA , Lexington (United States)
Type
Published Article
Journal
Plant Molecular Biology
Publisher
Springer Netherlands
Publication Date
Oct 06, 2015
Volume
89
Issue
4-5
Pages
451–462
Identifiers
DOI: 10.1007/s11103-015-0381-3
Source
Springer Nature
Keywords
License
Yellow

Abstract

Ginkgo biloba is one of the oldest living tree species and has been extensively investigated as a source of bioactive natural compounds, including bioactive flavonoids, diterpene lactones, terpenoids and polysaccharides which accumulate in foliar tissues. Despite this chemical diversity, relatively few enzymes associated with any biosynthetic pathway from ginkgo have been characterized to date. In the present work, predicted transcripts potentially encoding enzymes associated with the biosynthesis of diterpenoid and terpenoid compounds, including putative terpene synthases, were first identified by mining publicly-available G. biloba RNA-seq data sets. Recombinant enzyme studies with two of the TPS-like sequences led to the identification of GbTPS1 and GbTPS2, encoding farnesol and bisabolene synthases, respectively. Additionally, the phylogenetic analysis revealed the two terpene synthase genes as primitive genes that might have evolved from an ancestral diterpene synthase.

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