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Investigating fluorescent dyes in fluorescence-assisted screenings

Authors
  • Jee, Joo-Eun
  • Lim, Jaehong
  • Hyun, Hoon
  • Oon, Jessica
  • Ong, Yong Siang
  • Massif, Cedrik
  • Chang, Young-Tae
  • Choi, Hak Soo
  • Lee, Su Seong
Publication Date
Jan 01, 2014
Source
[email protected]
Keywords
License
Unknown
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Abstract

Screening of bead-based peptide libraries against fluorescent dye-labeled target proteins was found to be significantly influenced by the dye characteristics. Commercially available red fluorescent dyes with net negative charges adversely showed strong interactions with library beads. The introduction of zwitterionic dyes significantly reduced the unwanted interactions, which sheds light upon using the right fluorescent probe for acquisition of reliable results in various fluorescence-assisted applications. / 1 / 1 / 4 / scie / scopus

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