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Investigating the endophytic bacterial diversity and community structures in seeds of genetically related maize (Zea mays L.) genotypes

Authors
  • Liu, Yang1
  • Yan, Hai1
  • Zhang, Xiaoxia2
  • Zhang, Ruyang3
  • Li, Miao1
  • Xu, Tianjun3
  • Yang, Fuzhen1
  • Zheng, Huajun4
  • Zhao, Jiuran3
  • 1 University of Science and Technology Beijing, Beijing, 100083, China , Beijing (China)
  • 2 Ministry of Agriculture, Beijing, 100081, China , Beijing (China)
  • 3 Beijing Academy of Agriculture and Forestry Sciences, Beijing, 100097, China , Beijing (China)
  • 4 Chinese National Human Genome Center at Shanghai, Shanghai, 201203, China , Shanghai (China)
Type
Published Article
Journal
3 Biotech
Publisher
Springer International Publishing
Publication Date
Jan 04, 2020
Volume
10
Issue
1
Identifiers
DOI: 10.1007/s13205-019-2034-8
Source
Springer Nature
Keywords
License
Yellow

Abstract

This research aimed to investigate the composition and diversity of endophytic bacterial community in seeds of four hybrid maize and their parental lines, which was used to reveal the potential relationship and association of endophytic bacteria between maize genotypes and their genetic relevance. High-throughput sequencing (HTS) technology showed that a total of 1419 OTUs (46.6%) were parental lines unique and 1052 OTUs (34.5%) were hybrid varieties unique, with only 575 core OTUs revealed in all the samples. Most OTUs belonged to Proteobacteria. Enterobacter (23.2%), Shigella (21.2%), Pseudomonas (15.8%) and Achromobacter (10.1%) were the major genera; the bacterial community composition and diversity of endophytic bacteria were inconsistent among different seed genotypes. Based on principal component analysis (PCA), the results referred that the endophytic composition of hybrid sample showed obvious correlation with their female parental lines, and in ‘Jingke968’ and ‘MC738’ with the same female line the endophytic community was more similar than other hybrid samples. This was the first ever use of HTS technology for investigating the endophytic bacterial diversity and community structures in seeds of genetically related maize genotypes, it was shown that, there were core microbes shared among all genotypes of seed samples, and the female parental line was more significant to impact on the composition of their hybrid seeds than male parental line. This study would provide scientific clues for the future research on the vertical transmission of endophytes among maize generations.

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