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Invasion of Salmonella enteritidis in avian intestinal epithelial cells in vitro is influenced by short-chain fatty acids.

Authors
  • Van Immerseel, F
  • De Buck, J
  • Pasmans, F
  • Velge, P
  • Bottreau, E
  • Fievez, V
  • Haesebrouck, F
  • Ducatelle, R
Type
Published Article
Journal
International Journal of Food Microbiology
Publisher
Elsevier
Publication Date
Aug 25, 2003
Volume
85
Issue
3
Pages
237–248
Identifiers
PMID: 12878382
Source
Medline
License
Unknown

Abstract

Fermentation reactions in the caeca of chickens, the predominant place for Salmonella colonization, result in high concentrations of short-chain fatty acids (SCFA). Thus Salmonella bacteria are in close contact with SCFA during their life cycle. A study was carried out to analyse the effects of SCFA on invasion of Salmonella enteritidis in an avian intestinal epithelial cell line. Preincubation of S. enteritidis for 4 h in growth media supplemented with various concentrations of propionate or butyrate resulted in decreased invasion compared to bacteria, preincubated in nonsupplemented media, and to bacteria, preincubated in media supplemented with formate or acetate. Incubation of the S. enteritidis bacteria in media supplemented with mixtures of SCFA mimicking the in vivo caecal concentrations resulted in increased invasion compared with butyrate-exposed bacteria, but equal invasion compared with nonexposed bacteria. Increasing the butyrate concentration in these mixtures did not modify invasion compared with the original mixtures.

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