Affordable Access

deepdyve-link
Publisher Website

Intravital two-photon imaging of the gastrointestinal tract.

Authors
  • Kolesnikov, Masha
  • Farache, Julia
  • Shakhar, Guy
Type
Published Article
Journal
Journal of Immunological Methods
Publisher
Elsevier
Publication Date
Jun 01, 2015
Volume
421
Pages
73–80
Identifiers
DOI: 10.1016/j.jim.2015.03.008
PMID: 25801674
Source
Medline
Keywords
License
Unknown

Abstract

Live imaging of the gastrointestinal tract with two-photon microscopy (TPM) has proven to be a useful tool for mucosal immunologists. It provides deep penetration of live tissues with reduced phototoxicity and photobleaching and thus excels in deciphering dynamic immunological processes that require cell motility and last minutes through hours. The few studies that employed this technique in the gut have uncovered new aspects of mucosal immunity. They focused mainly on adaptive immunity in the small intestine and exposed the details of important interactions among several epithelial and hematopoietic cell types. TPM can be employed either on explanted tissue or intravitally, as has been practiced in our lab. Intravital TPM preserves physiological conditions more faithfully, but it is a demanding technique that requires dedicated personnel. To achieve success, the peristaltic motility of the intestine must be curbed, surgical and photonic damage must be minimized, and tissue degradation must be delayed and controlled for. Here we briefly review published studies that employed intravital TPM in the gut, describe our own technique for imaging the intestinal Peyer's patches (PPs) and villi, and present some observations we made using this technique.

Report this publication

Statistics

Seen <100 times