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Intravital fluorescence microscopy in pulmonary research.

Authors
  • St Croix, Claudette M
  • Leelavanichkul, Karanee
  • Watkins, Simon C
Type
Published Article
Journal
Advanced Drug Delivery Reviews
Publisher
Elsevier
Publication Date
Sep 15, 2006
Volume
58
Issue
7
Pages
834–840
Identifiers
PMID: 16996641
Source
Medline
License
Unknown

Abstract

Over the last several years, microscopy as a scientific tool has reinvented itself evolving from a group of principally descriptive methodologies to encompass a wide range of primary tools and techniques to investigate the molecular organization of organs, tissues and cells. Advances in microscope and camera design, fluorescent dye technology, the development of fluorescent proteins as well as the advent of inexpensive powerful computers, has led to the feasibility of simultaneous sub micron resolution and quantitation of multiple concurrent molecular markers for both protein and DNA. Confocal microscopy has allowed optical sectioning and reconstruction of tissues in three dimensions. Finally, the development of multiphoton methodologies as an extension of optical sectioning microscopy has further improved the potential utility of this technology when examining living or light scattering tissues such as the lung. In order to illustrate the utility of two-photon methods in pulmonary biology, we present the application of this approach to the study of cellular trafficking in situ and to the study of pulmonary vasoregulation in an ex vivo rodent model.

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