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[Intravital bone imaging ~Osteocyte.]

Authors
  • Sano, Hiroshige1
  • Kondo, Naoki2
  • Endo, Naoto2
  • 1 Division of Orthopedic Surgery, Department of Regenerative and Transplant Medicine, Niigata University Graduate School of Medical and Dental Sciences/Department of Immunology and Cell Biology, Graduate School of Medicine and Frontier Biosciences, Osaka University, Japan/Bone Research Group, Department of Medicine, University of Cambridge, UK. , (Japan)
  • 2 Division of Orthopedic Surgery, Department of Regenerative and Transplant Medicine, Niigata University Graduate School of Medical and Dental Sciences, Japan. , (Japan)
Type
Published Article
Journal
Clinical calcium
Publication Date
Jan 01, 2018
Volume
28
Issue
2
Pages
223–230
Identifiers
DOI: CliCa1802223230
PMID: 29371488
Source
Medline
License
Unknown

Abstract

Osteocytes are the most abundant cellular component of bone and have been considered dormant until recent evidence has demonstrated their critical roles in bone homeostasis and endocrine regulation. Until now the location of osteocytes within mineralized bone has restricted experimental access, especially in vivo. Here, intravital bone imaging by two-photon excitation microscopy allowed us to directly visualize the osteocytic lacuno-canalicular system. We demonstrated that sciatic neurectomy causes significant acidification around osteocytic lacunae and enlargement of lacuno-canalicular areas. These results show that two-photon intravital microscopy is useful for analysis of osteocytes in vivo.

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