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Intracellular distribution of pseudorabies virus UL2 and detection of its nuclear import mechanism

Authors
  • Li, Meili1, 2
  • Xu, Zuo1, 2
  • Zou, Xingmei1, 2
  • Wang, Yuanfang1, 2
  • Li, Yiwen1, 2
  • Ou, Xiaowen1, 2
  • Deng, Yangxi1, 2
  • Guo, Yingjie1, 2
  • Gan, Weidong1, 2
  • Chen, Daixiong1, 2
  • Peng, Tao3, 4
  • Xiao, Jing3
  • Cai, Mingsheng1, 2
  • 1 Second Affiliated Hospital of Guangzhou Medical University, China , (China)
  • 2 Guangzhou Medical University, No. 250 Changgang Dong Road, Haizhu District , (China)
  • 3 Guangzhou Medical University, Xinzao Town, Panyu , (China)
  • 4 South China Vaccine Corporation Limited, Guangzhou Science Park, China , (China)
Type
Published Article
Journal
Biological Chemistry
Publisher
Walter de Gruyter GmbH
Publication Date
Oct 30, 2019
Volume
401
Issue
2
Pages
309–317
Identifiers
DOI: 10.1515/hsz-2019-0311
Source
De Gruyter
Keywords
License
Yellow

Abstract

Pseudorabies virus (PRV) UL2 (pUL2) is a multifunctional protein, which is homologous with herpes simplex virus 1 early protein UL2 (hUL2) and crucial for the viral propagation. Yet, how pUL2 executes its roles in the viral life cycle remain inadequately understood. In order to uncover its effect on the procedure of PRV infection, investigation was performed to examine the subcellular distribution of pUL2 and establish its trafficking mechanism. In the present study, enhanced yellow fluorescent protein or Myc tag fused pUL2 was transiently overexpressed in transfected cells and exhibited an absolutely nuclear accumulation without the existence of other PRV proteins. Additionally, the nuclear trafficking of pUL2 was proved to rely on Ran-, transportin-1, importin β1, importin α1, α3 and α5. Accordingly, these data will benefit the knowledge of pUL2-mediated biological effects in PRV infection cycle.

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