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Intracellular distribution of CPT-11 in CPT-11-resistant cells with confocal laser scanning microscopy.

Authors
  • Oyama, H
  • Nagane, M
  • Shibui, S
  • Nomura, K
  • Mukai, K
Type
Published Article
Journal
Japanese journal of clinical oncology
Publication Date
Oct 01, 1992
Volume
22
Issue
5
Pages
331–334
Identifiers
PMID: 1335095
Source
Medline
License
Unknown

Abstract

CPT-11, 7-ethyl-10-[4-(1-piperidino)-1-piperidino]carbonyloxy camptothecin, is a well-known DNA topoisomerase I inhibitor. SN-38 is a metabolite of this compound. Both emit fluorescence when activated by a laser beam. With a confocal laser scanning microscope (CLSM), we determined the intracellular distribution of CPT-11 and SN-38 and the chronological changes in drug-treated PC-7, a cell line of human non-small cell lung cancer, and its CPT-11 resistant variant, PC-7/CPT cells. There were many more granules in the cytoplasm in PC-7/CPT than in the parent cell line (PC-7). The granule formation of the resistant cell could indicate a different drug metabolism in the cytoplasm from that of the parent cell. This technique would provide a new way of investigating the mechanism of resistance of cancer cells to anticancer drugs.

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