Aims. To study the intra-and interobserver reproducibility of Sydney System amongst pathologists in Cameroon as Sydney System gradation has not gained enough confidence in African pathologists. Methods. We performed a descriptive study including 100 patients who benefited from gastric biopsy by endoscopy. These biopsy specimens were stained with hematein and eosin and modified Giemsa, and read independently using the same microscope by two pathologists with four years experience and no experience with the updated Sydney System. Gastritis was graded according to the updated Sydney System. Levels of intra-and interobserver reproducibility were assessed using the unweighted kappa coefficient. Results. The intraobserver reproducibility of gradation of Helicobacter pylori density, activity, chronic inflammation, atrophy, and intestinal metaplasia showed respective values of kappa: 0.63, 0.34, 0.61, 0.48, and 0.82 for one observer against 0.42, 0.005, 0.41, 0.31, and 0.72 for the other. Interobserver reproducibility kappa values were, respectively, 0.41, 0.18, 0.57, 0.58, and 0.82. Conclusion. Results are encouraging but experience in the updated Sydney System should be improved. The later should be introduced as a means to grade and classify gastritis in Cameroon and African countries.