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Interactive effects of chronic stress and a high-sucrose diet on nonalcoholic fatty liver in young adult male rats.

Authors
  • Corona-Pérez, Adriana1
  • Díaz-Muñoz, Mauricio2
  • Cuevas-Romero, Estela3
  • Luna-Moreno, Dalia4
  • Valente-Godínez, Héctor2
  • Vázquez-Martínez, Olivia2
  • Martínez-Gómez, Margarita3, 5
  • Rodríguez-Antolín, Jorge3
  • Nicolás-Toledo, Leticia3
  • 1 a Doctorado en Ciencias Biológicas , Universidad Autónoma de Tlaxcala , Tlaxcala , México.
  • 2 b Departamento de Neurobiología Celular y Molecular, Instituto de Neurobiología , Campus UNAM-Juriquilla, Universidad Nacional Autónoma de México , Querétaro , México.
  • 3 c Centro Tlaxcala de Biología de la Conducta , Universidad Autónoma de Tlaxcala , Tlaxcala , México.
  • 4 d CONACyT-Facultad de Ciencias Naturales , Universidad Autónoma de Querétaro , Querétaro , México.
  • 5 e Departamento de Biología Celular y Fisiología, Instituto de Investigaciones Biomédicas , Universidad Nacional Autónoma de México , México-CDMX , México.
Type
Published Article
Journal
Stress (Amsterdam, Netherlands)
Publication Date
Nov 01, 2017
Volume
20
Issue
6
Pages
608–617
Identifiers
DOI: 10.1080/10253890.2017.1381840
PMID: 28969511
Source
Medline
Keywords
License
Unknown

Abstract

Glucocorticoids have been implicated in nonalcoholic fatty liver diseases (NAFLD). The influence of a palatable diet on the response to stress is controversial. This study explored whether a high-sucrose diet could protect from hepatic steatosis induced by chronic restraint stress in young adult rats. Male Wistar rats aged 21 days were allocated into four groups (n = 6-8 per group): control, chronic restraint stress, 30% sucrose diet, and 30% sucrose diet plus chronic restraint stress. After being exposed to either tap water or sucrose solution during eight weeks, half of the rats belonging to each group were subject or not to repeated restraint stress (1 h per day, 5 days per week) during four weeks. Triacylglycerol (TAG), oxidative stress, activity of 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD-1), infiltration of immune cells, and glycogen amount in the liver were quantified. Serum concentrations of corticosterone and testosterone were also measured. The stressed group showed normal serum concentrations of corticosterone and did not have hepatic steatosis. However, this group showed increased glycogen, inflammation, mild fibrosis, oxidative stress, and a high activity of 11β-HSD-1 in the liver. The group exposed to the high-sucrose diet had lower concentrations of corticosterone, hepatic steatosis and moderate fibrosis. The group subject to high-sucrose diet plus chronic restraint stress showed low concentrations of corticosterone, hepatic steatosis, oxidative stress, and high concentrations of testosterone. Thus, restraint stress and a high-sucrose diet each generate different components of nonalcoholic fatty liver in young adult rats. The combination of both the factors could promote a faster development of NAFLD.

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