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Interaction of an immature seed-specific trans-acting factor with the 5' upstream region of a rice glutelin gene.

Authors
  • Takaiwa, F
  • Oono, K
Type
Published Article
Journal
Molecular & general genetics : MGG
Publication Date
Nov 01, 1990
Volume
224
Issue
2
Pages
289–293
Identifiers
PMID: 2277646
Source
Medline
License
Unknown

Abstract

The 5' flanking region of a glutelin gene was analyzed for interactions with nuclear proteins from immature rice seed. The specific region between positions -272 and -99 was shown to interact with nuclear proteins from immature seeds, but not with those of leaves and roots. Methylation interference experiments revealed that one factor interacted with a specific sequence element between positions -130 and -120 relative to the transcriptional start site. The sequence specificity of this DNA-protein interaction was confirmed by competition experiments using synthetic oligonucleotides. By using a synthetic oligonucleotide as a probe it was also shown that the binding activity was closely correlated with the mRNA levels of this gene during seed maturation.

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