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[Interaction of EcoRII restriction and modification enzymes with synthetic DNA fragments. Determination of the size of EcoRII binding site].

Authors
  • Vinogradova, M V
  • Gromova, E S
  • Kosykh, V G
  • Bur'ianov, Ia I
  • Shabarova, Z A
Type
Published Article
Journal
Molekuliarnaia biologiia
Publication Date
Jan 01, 1990
Volume
24
Issue
3
Pages
847–850
Identifiers
PMID: 2402242
Source
Medline
License
Unknown

Abstract

EcoRII restriction endonuclease binding site has been determined on the basis of comparison of the binding parameters of the enzyme with synthetic DNA-duplexes of concatemer type containing a different number of EcoRII recognition sites. It has been shown that it consists of 21 +/- 1 base pairs.

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