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The interaction of artemisinin with malarial hemozoin.

Authors
  • Hong, Y L
  • Yang, Y Z
  • Meshnick, S R
Type
Published Article
Journal
Molecular and Biochemical Parasitology
Publisher
Elsevier
Publication Date
Jan 01, 1994
Volume
63
Issue
1
Pages
121–128
Identifiers
PMID: 8183310
Source
Medline
License
Unknown

Abstract

[14C]Artemisinin was taken up by Plasmodium falciparum in culture and concentrated in hemozoin. In vitro, hemin and artemisinin were found to undergo a chemical reaction forming two major products which were isolated by high-performance liquid chromatography (HPLC). The m/z values of the two products were 856 and 871. Thin-layer chromatography (TLC) and HPLC of hemozoin isolated from [14C]artemisinin-treated parasites showed that the majority of the hemozoin-associated radioactivity comigrated with the synthetic adducts. When [14C]artemisinin was incubated with isolated hemozoin, [14C]artemisinin disappeared from the solution in a time-dependent manner. Some of the radioactivity present in the treated hemozoin also comigrated with the adducts on TLC. Thus, artemisinin appears to react covalently with heme in malaria hemozoin both in vitro and in situ.

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