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Inter- and intra-strain genomic variation in equine herpesvirus type 1 isolates.

Authors
Type
Published Article
Journal
Archives of virology
Publication Date
Volume
134
Issue
1-2
Pages
169–178
Identifiers
PMID: 8279952
Source
Medline
License
Unknown

Abstract

Restriction enzyme digests of DNA from 22 unselected isolates of EHV-1 were analysed by hybridization with cloned DNA fragments covering the genome. In addition to a small amount of inter-strain variation, heterogeneity within strains was observed, caused by loss of specific restriction endonuclease sites in the DNA of a proportion of the virus particles of any one stock. Fifteen strains demonstrated the same intra-strain variation involving loss of the BamHI L-M site which was shown to lie within coding sequence for the large subunit of ribonucleotide reductase. This particular mutation may therefore be selected for by passage in RK13 cells.

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