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Integrin α3 is involved in non-enveloped hepatitis E virus infection.

Authors
  • Shiota, Tomoyuki1
  • Li, Tian-Cheng1
  • Nishimura, Yorihiro1
  • Yoshizaki, Sayaka1
  • Sugiyama, Ryuichi1
  • Shimojima, Masayuki2
  • Saijo, Masayuki2
  • Shimizu, Hiroyuki1
  • Suzuki, Ryosuke1
  • Wakita, Takaji1
  • Muramatsu, Masamichi1
  • Ishii, Koji3
  • 1 Department of Virology II, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashi-murayama, Tokyo, 208-0011, Japan. , (Japan)
  • 2 Department of Virology I, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashi-murayama, Tokyo, 208-0011, Japan. , (Japan)
  • 3 Department of Quality Assurance and Radiological Protection, National Institute of Infectious Diseases, Gakuen 4-7-1, Musashi-murayama, Tokyo, 208-0011, Japan. Electronic address: [email protected] , (Japan)
Type
Published Article
Journal
Virology
Publisher
Elsevier
Publication Date
Jul 30, 2019
Volume
536
Pages
119–124
Identifiers
DOI: 10.1016/j.virol.2019.07.025
PMID: 31421623
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

Hepatitis E virus (HEV) causes acute and fulminant hepatitis worldwide. Although enveloped (e) and non-enveloped (ne) forms of HEV have been discovered, host factors involved in infection, including receptors, remain to be elucidated. Here, we identified integrin α3 (encoded by ITGA3), a protein that binds and responds to the extracellular matrix, as an essential host factor for HEV infection. Integrin α3 expression was lower in four HEV-non-permissive cell subclones than in an HEV-permissive subclone. ITGA3 knockout cells lost HEV permissibility, suggesting that integrin α3 is critical for HEV infection. Stable expression of integrin α3 in an HEV-non-permissive subclone provided permissibility only to infection by neHEV; expression of integrin α3 lacking the ectodomain did not. Direct interaction between neHEV and the integrin α3 ectodomain was confirmed by co-precipitation using a soluble integrin α3-Fc. These results strongly suggest that integrin α3 is a key molecule for cellular attachment and entry of neHEV. Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.

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