Affordable Access

deepdyve-link
Publisher Website

Integration of Neurons Derived from Transplanted BDNF-Overexpressing Neural Stem Cells into Circuit of the Irradiated Hippocampus.

Authors
  • Zhang, Qixian1, 2, 3
  • Zhong, Xue1, 2, 3
  • Zhang, Jie1, 2, 3
  • Zhu, Hao4
  • Cai, Shang1, 2, 3
  • Zhang, Junjun1, 2, 3
  • Cao, Yi5
  • Tian, Ye1, 2, 3
  • 1 The Second Affiliated Hospital of Soochow University, Soochow University, Suzhou, Jiangsu, China. , (China)
  • 2 Institute of Radiotherapy and Oncology, Soochow University, Suzhou, Jiangsu, China. , (China)
  • 3 Suzhou Key Laboratory for Radiation Oncology, Suzhou, Jiangsu, China. , (China)
  • 4 Institute of Neuroscience, Soochow University, Suzhou, Jiangsu, China. , (China)
  • 5 School of Public Health, Soochow University, Suzhou, Jiangsu, China. , (China)
Type
Published Article
Journal
Radiation Research
Publisher
BioOne (Radiation Research Society)
Publication Date
Sep 01, 2019
Volume
192
Issue
3
Pages
345–351
Identifiers
DOI: 10.1667/RR15409.1
PMID: 31295088
Source
Medline
Language
English
License
Unknown

Abstract

Stem cell transplantation is thought to be an effective method for radiation-induced cognitive dysfunction. However, there have been few studies performed to determine whether transplanted stem cells can integrate into hippocampus circuits. Brain-derived neurotrophic factor (BDNF) plays a critical role in brain development. Therefore, we investigated the differentiation and integration of brain-derived neurotrophic factor overexpressing neural stem cells (NSCs). We observed that these transplanted cells migrated to the subgranular zone of irradiated rats at 4 weeks after transplantation. However, control neural stem cells were disordered, distributing in the irradiated hippocampus, and showed greater astroglia differentiation tendency. Retrograde monosynaptic tracing showed that neurons derived from transplanted brain-derived neurotrophic factor overexpressing neural stem cells integrated into the circuit better than those from control cells. Brain-derived neurotrophic factor overexpressing neural stem cells s promoted the expression of brain-derived neurotrophic factor and nerve growth factor and reduced the number of activated microglia caused by radiation. Transplanted brain-derived neurotrophic factor overexpressing neural stem cells failed to improve radiation-induced cognitive dysfunction. These results indicate that brain-derived neurotrophic factor overexpressing neural stem cells suffered less from changed microenvironment after irradiation and possessed the ability to improve the host niche. Neurons derived from Brain-derived neurotrophic factor overexpressing neural stem cells showed the integration potency in the irradiated hippocampus.

Report this publication

Statistics

Seen <100 times