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Integration of human immunodeficiency virus type 1 DNA in vitro.

Authors
  • Farnet, C M
  • Haseltine, W A
Type
Published Article
Journal
Proceedings of the National Academy of Sciences of the United States of America
Publication Date
Jun 01, 1990
Volume
87
Issue
11
Pages
4164–4168
Identifiers
PMID: 2349226
Source
Medline
License
Unknown

Abstract

A highly efficient cell-free system for the integration of human immunodeficiency virus type 1 DNA is described. Linear viral DNA synthesis occurs in the cytoplasm of newly infected cells, reaching peak levels 4 hr after infection. The linear viral DNA molecules present in cytoplasmic extracts are capable of integrating into heterologous DNA targets in vitro. The viral DNA resides in a high molecular weight nucleoprotein structure that can be separated from the bulk of cellular protein and nucleic acid without a detectable decrease in the ability to integrate in vitro.

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