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Integrated version of reverse two-hybrid system for the postproteomic era.

Authors
  • Endoh, Hideki
  • Vincent, Sylvie
  • Yves Jacob
  • Réal, Eléonore
  • Walhout, Albertha J M
  • Vidal, Marc
Type
Published Article
Journal
G Protein Coupled Receptors - Structure
Publisher
Elsevier BV
Publication Date
Jun 12, 2002
Volume
350
Pages
525–545
Identifiers
DOI: 10.1016/s0076-6879(02)50983-6
PMID: 12073334
Source
USPC - SET - SVS
License
White

Abstract

Functional genomic databases provide information for many of the genes predicted from genome and expressed sequence tags (ESTs) sequencing projects. These databases should gradually relieve molecular biologists from the tedious and repetitive steps involved in conventional gene cloning and basic protein characterization. This in turn might help extend studies primarily focused on the function of proteins toward more precisely deciphering the function of interactions between proteins. This chapter describes an improved version of the yeast reverse two-hybrid system to generate and characterize interaction-defective alleles (IDAs) that can be used to study the function of physical interactions between proteins. Comprehensive interactome mapping projects using large-scale yeast two-hybrid systems are underway for Saccharomyces cerevisiae and Caenorhabditis elegans. Typically two-hybrid interactome maps consist of large lists of potential binary interactions made available on the World Wide Web. These maps should provide additional layers of information on various aspects of protein function.

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