Integrated silicon microfluidic chip for picoliter-scale analyte segmentation and microscale printing for mass spectrometry imaging.
- Authors
- Type
- Published Article
- Journal
- Lab on a Chip
- Publisher
- The Royal Society of Chemistry
- Publication Date
- Dec 20, 2022
- Volume
- 23
- Issue
- 1
- Pages
- 72–80
- Identifiers
- DOI: 10.1039/d2lc00688j
- PMID: 36477760
- Source
- Medline
- Language
- English
- License
- Unknown
Abstract
A silicon single-chip microfluidics system that integrates microscale fluidic channels, an analyte segmentation device, and a nozzle for electrohydrodynamic-assisted printing is designed for hyphenation with MALDI mass spectrometry (MS) imaging. A miniaturized T-junction segments analytes into monodisperse picoliter oil-isolated compartments. The printing nozzle deposits generated droplets one-by-one into an array on a conductive substrate without splitting or coalescing. Virtually single-shot MS analysis is enabled due to the ultrasmall droplet volumes and highly localized printing. The signal-to-noise ratio indicates that detection limits at the attomole level are achieved for γ-aminobutyric acid.
