Affordable Access

Integrated gene analyses of de novo variants from 46,612 trios with autism and developmental disorders

Authors
  • Wang, Tianyun
  • Kim, Chang N
  • Bakken, Trygve E
  • Gillentine, Madelyn A
  • Henning, Barbara
  • Mao, Yafei
  • Gilissen, Christian
  • Consortium, The SPARK
  • Nowakowski, Tomasz J
  • Eichler, Evan E
  • Acampado, John
  • Ace, Andrea J
  • Amatya, Alpha
  • Astrovskaya, Irina
  • Bashar, Asif
  • Brooks, Elizabeth
  • Butler, Martin E
  • Cartner, Lindsey A
  • Chin, Wubin
  • Chung, Wendy K
  • And 79 more
Publication Date
Nov 15, 2022
Source
eScholarship - University of California
Keywords
License
Unknown
External links

Abstract

Most genetic studies consider autism spectrum disorder (ASD) and developmental disorder (DD) separately despite overwhelming comorbidity and shared genetic etiology. Here, we analyzed de novo variants (DNVs) from 15,560 ASD (6,557 from SPARK) and 31,052 DD trios independently and also combined as broader neurodevelopmental disorders (NDDs) using three models. We identify 615 NDD candidate genes (false discovery rate [FDR] < 0.05) supported by ≥1 models, including 138 reaching Bonferroni exome-wide significance (P < 3.64e-7) in all models. The genes group into five functional networks associating with different brain developmental lineages based on single-cell nuclei transcriptomic data. We find no evidence for ASD-specific genes in contrast to 18 genes significantly enriched for DD. There are 53 genes that show mutational bias, including enrichments for missense (n = 41) or truncating (n = 12) DNVs. We also find 10 genes with evidence of male- or female-bias enrichment, including 4 X chromosome genes with significant female burden (DDX3X, MECP2, WDR45, and HDAC8). This large-scale integrative analysis identifies candidates and functional subsets of NDD genes.

Report this publication

Statistics

Seen <100 times