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An integrated fritless column for on-chip capillary electrochromatography with conventional stationary phases.

Authors
  • Ceriotti, Laura
  • de, RooijNicoF
  • Verpoorte, Elisabeth
Type
Published Article
Journal
Analytical chemistry
Publication Date
Feb 01, 2002
Volume
74
Issue
3
Pages
639–647
Identifiers
PMID: 11838686
Source
Medline
License
Unknown

Abstract

A new polymer device for use with conventional particulate stationary phases for on-chip, fritless, capillary electrochromatography (CEC) has been realized. The structure includes an injector and a tapered column in which the particles of the stationary phase are retained and stabilized. The chips were easily fabricated in poly(dimethylsiloxane) using deep-reactive-ion-etched silicon masters, and tested using a capillary electrophoretic separation of FITC-labeled amino acids. To perform CEC, the separation channel was packed using a vacuum with 3-microm, octadecylsilanized silica microspheres. The packing was stabilized in the column by a thermal treatment, and its stability and quality were evaluated using in-column indirect fluorescence detection. The effects of voltage on electro-osmotic flow and on efficiency were investigated, and the separation of two neutral compounds was achieved in less than 15 s.

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