Affordable Access

deepdyve-link
Publisher Website

In-Silico Analysis of Chromatin Modifiers and Profiling of Histone Deacetylases (HDAC's) in Human Oral Cancer

Authors
  • Sajnani, Anand K.1
  • Shah, Sanket G.1, 1
  • Rashid, Mudasir1, 1
  • Natu, Abhiram1, 1
  • Gera, Poonam B.1
  • Gupta, Sanjay1, 1
  • 1 . , (Qatar)
Type
Published Article
Journal
Chonnam Medical Journal
Publisher
Chonnam National University Medical School
Publication Date
Sep 24, 2021
Volume
57
Issue
3
Pages
176–184
Identifiers
DOI: 10.4068/cmj.2021.57.3.176
PMID: 34621637
PMCID: PMC8485086
Source
PubMed Central
Keywords
Disciplines
  • Original Article
License
Unknown

Abstract

Histone modifications have been demonstrated to play a significant role in oral squamous cell carcinoma (OSCC) epigenetic regulation. An in-silico analysis of The Cancer Genome Atlas (TCGA) of various histone acetyl transferases (HATs) and histone deacetylases (HDACs) suggested that HATs do not differ between normal and tumor samples whereas HDAC2 and HDAC1 change maximally and marginally respectively between normal and tumor patients with no change being noted in HDAC6 expression. Hence, this investigation was carried out to validate the expression states of HDAC 1, 2 and 6 mRNAs in buccal mucosa and tongue SCC samples in an Indian cohort. Buccal mucosa and tongue squamous cell carcinoma tissues with intact histopathology were processed for RNA isolation followed by cDNA synthesis which was then subjected to q-PCR for HDACs. The average RNA yield of the tongue tissue sample was ∼2 µg/mg of tissue and the A260/280 ratios were between 2.03 and 2.06. The average RNA yield of buccal mucosa tissue sample was ∼1 µg/mg of tissue and the A260/280 ratio were between 2.00 and 2.08. We have demonstrated that HDAC2 was overexpressed in tongue and buccal mucosa samples. Over-expression of HDAC2 imply potential use of HDACi along with standard chemotherapeutic drug in oral cancer treatment.

Report this publication

Statistics

Seen <100 times