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Insights on PRAME and osteosarcoma by means of gene expression profiling

Authors
  • Toledo, Sílvia Regina Caminada1, 2
  • Zago, Marco Antonio3
  • Oliveira, Indhira Dias1, 2
  • Proto-Siqueira, Rodrigo3
  • Okamoto, Oswaldo K.4
  • Severino, Patricia5
  • Vêncio, Ricardo Z. N.6
  • Gamba, Francine Tesser1, 2
  • Silva, Wilson A.3, 6
  • Moreira-Filho, Carlos A.7
  • Torre, Cristiane A. Dalla1, 2
  • Alves, Maria Tereza Seixas1, 8
  • Garcia-Filho, Reynaldo J.1, 9
  • Simpson, Andrew J. G.10
  • Petrilli, Antonio Sergio1
  • 1 Pediatric Oncology Institute-GRAACC (Grupo de Apoio ao Adolescente e à Criança com Câncer)/UNIFESP (Federal University of São Paulo), Department of Pediatrics, Rua Botucatu no. 743, Floor 8, Genetics Laboratory, Vila Clementino, São Paulo, SP, 04023-062, Brazil , São Paulo (Brazil)
  • 2 Federal University of São Paulo, Department of Morphology and Genetics, São Paulo, SP, Brazil , São Paulo (Brazil)
  • 3 Faculty of Medicine of Ribeirão Preto-University of São Paulo, Department of Clinical Medicine and Center of Cell-based Therapy, Ribeirão Preto, SP, Brazil , Ribeirão Preto (Brazil)
  • 4 University of São Paulo, Department of Genetics and Evolutionary Biology, São Paulo, SP, Brazil , São Paulo (Brazil)
  • 5 Albert Einstein Research and Education Institute, São Paulo, SP, Brazil , São Paulo (Brazil)
  • 6 University of São Paulo, Genetics Department, Ribeirão Preto, SP, Brazil , Ribeirão Preto (Brazil)
  • 7 University of São Paulo, Pediatrics Department, São Paulo, SP, Brazil , São Paulo (Brazil)
  • 8 Federal University of São Paulo, Department of Pathology, São Paulo, SP, Brazil , São Paulo (Brazil)
  • 9 Federal University of São Paulo, Department of Orthopedic Surgery and Traumatology, São Paulo, SP, Brazil , São Paulo (Brazil)
  • 10 Ludwig Institute, New York, NY, USA , New York (United States)
Type
Published Article
Journal
Journal of Orthopaedic Science
Publisher
Elsevier
Publication Date
Jun 21, 2011
Volume
16
Issue
4
Pages
458–466
Identifiers
DOI: 10.1007/s00776-011-0106-7
Source
Springer Nature
Keywords
License
Yellow

Abstract

BackgroundOsteosarcoma (OS) is the most frequent bone tumor in children and adolescents. Tumor antigens are encoded by genes that are expressed in many types of solid tumors but are silent in normal tissues, with the exception of placenta and male germ-line cells. It has been proposed that antigen tumors are potential tumor markers.ObjectivesThe premise of this study is that the identification of novel OS-associated transcripts will lead to a better understanding of the events involved in OS pathogenesis and biology.MethodsWe analyzed the expression of a panel of seven tumor antigens in OS samples to identify possible tumor markers. After selecting the tumor antigen expressed in most samples of the panel, gene expression profiling was used to identify osteosarcoma-associated molecular alterations. A microarray was employed because of its ability to accurately produce comprehensive expression profiles.ResultsPRAME was identified as the tumor antigen expressed in most OS samples; it was detected in 68% of the cases. Microarray results showed differences in expression for genes functioning in cell signaling and adhesion as well as extracellular matrix-related genes, implying that such tumors could indeed differ in regard to distinct patterns of tumorigenesis.ConclusionsThe hypothesis inferred in this study was gathered mostly from available data concerning other kinds of tumors. There is circumstantial evidence that PRAME expression might be related to distinct patterns of tumorigenesis. Further investigation is needed to validate the differential expression of genes belonging to tumorigenesis-related pathways in PRAME-positive and PRAME-negative tumors.

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