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Insights into the interactions between enzyme and co-solvents: stability and activity of stem bromelain.

Authors
  • Rani, Anjeeta1
  • Venkatesu, Pannuru2
  • 1 Department of Chemistry, University of Delhi, Delhi 110 007, India. , (India)
  • 2 Department of Chemistry, University of Delhi, Delhi 110 007, India. Electronic address: [email protected] , (India)
Type
Published Article
Journal
International journal of biological macromolecules
Publication Date
Feb 01, 2015
Volume
73
Pages
189–201
Identifiers
DOI: 10.1016/j.ijbiomac.2014.10.068
PMID: 25434803
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

In present study, an attempt is made to elucidate the effects of various naturally occurring osmolytes and denaturants on BM at pH 7.0. The effects of the varying concentrations of glycerol, sorbitol, sucrose, trehalose, urea and guanidinium chloride (GdnHCl) on structure, stability and activity of BM are explored by fluorescence spectroscopy, circular dichroism (CD), UV-vis spectroscopy and sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Our experimental observations reveal that glycerol and sorbitol are acting as stabilizers at all concentrations while sucrose and trehalose are found to be destabilizers at lower concentrations, however, acted as stabilizers at higher concentrations. On the other hand, urea and GdnHCl are denaturants except at lower concentrations. There is a direct relationship between activity and conformational stability as the activity data are found to be in accordance with conformational stability parameters (ΔGu, Tm, ΔCp) and BM profile on SDS-PAGE. Copyright © 2014 Elsevier B.V. All rights reserved.

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