Affordable Access

Insertion of natural intron 6a-6b into a human cDNA-derived gene therapy vector for cystic fibrosis improves plasmid stability and permits facile RNA/DNA discrimination.

Authors
  • Boyd, A C
  • Popp, F
  • Michaelis, U
  • Davidson, H
  • Davidson-Smith, H
  • Doherty, A
  • McLachlan, G
  • Porteous, D J
  • Seeber, S
Type
Published Article
Journal
The journal of gene medicine
Publication Date
Jan 01, 1999
Volume
1
Issue
5
Pages
312–321
Identifiers
PMID: 10738548
Source
Medline
License
Unknown

Abstract

Instability of a plasmid vector for gene therapy has been minimized by rational modification. The introduction of an intron for this purpose offers the additional advantage of providing a discriminatory RT-PCR assay.

Report this publication

Statistics

Seen <100 times