Affordable Access

deepdyve-link
Publisher Website

Inhibitory effect of nicardipine on rotenone‑induced apoptosis in SH‑SY5Y human neuroblastoma cells.

Authors
  • Park, Hae Jeong
  • Kim, Hak-Jae
Type
Published Article
Journal
Molecular Medicine Reports
Publisher
Spandidos Publications
Publication Date
Mar 01, 2013
Volume
7
Issue
3
Pages
941–946
Identifiers
DOI: 10.3892/mmr.2013.1260
PMID: 23291720
Source
Medline
License
Unknown

Abstract

Previous studies have demonstrated that calcium channel blockers have protective effects on damaged brains. In the present study, the protective effect of the calcium channel blocker nicardipine against rotenone‑induced apoptosis in SH‑SY5Y human neuroblastoma cells was investigated, focusing on mitogen‑activated protein kinases (MAPKs) and caspase (CASP)‑mediated apoptotic events. Nicardipine was found to decrease rotenone‑induced apoptosis through 4,6‑diamidino‑2‑phenylindole staining and the terminal deoxynucleotidyl transferase‑mediated dUTP nick end‑labeling assay. In addition, nicardipine was identified to inhibit rotenone‑induced elevation of intracellular Ca2+ concentration measured using the Fluo‑4 AM fluorescent dye. Rotenone increased phosphorylation of c‑Jun NH2‑terminal kinase (JNK) and p38 MAPK, whereas nicardipine blocked these increases. Nicardipine also prevented downregulation of B‑cell CLL/lymphoma 2 and upregulation of Bcl2‑associated X protein by rotenone. Furthermore, nicardipine abrogated cleavage of CASP9 and 3 and poly (ADP‑ribose) polymerase‑1 by rotenone and CASP3 enzyme activity in rotenone‑treated cells. These results indicate that nicardipine exerts a protective effect against rotenone‑induced apoptosis in SH‑SY5Y cells, inhibiting phosphorylation of JNK and p38 MAPK and activation of CASPs.

Report this publication

Statistics

Seen <100 times