The anti-neovascularization treatment is one of the effective strategies for tumor molecular target therapy. At present, the target and effect of the anti-neovascularization treatment is limited, and it is urgent to establish a new vascular targeting strategy to effectively treat tumors. In this work, we used high intensity focused ultrasound (HIFU) combined with targeted microbubbles to establish a molecular targeted ultrasound response microbubble for neovascular cells. Furthermore, the effects of drug loaded microbubbles on neovascularization and tumor cells were studied. The tumor vascular targeted and ultrasound-responsive microbubbles of [email protected] were prepared by the thin-film dispersion method. The size and zeta potential of [email protected] was about 1248 nm and -9.1 mV. [email protected] released 5-FU showed an ultrasound-responsive manner, and had better vascular-targeting ability. Furthermore, the [email protected] showed the strongest cytotoxic effect on HUVECs or HepG-2 cells and can be effectively internalized into the HUVECs cells. Thus, [email protected] combined with HIFU can be considered as a potential method for antitumor angiogenesis in the future. Copyright © 2020. Published by Elsevier Ltd.