Affordable Access

deepdyve-link
Publisher Website

Inhibition of Proliferation in U937 Cells Treated by Blue Light Irradiation and Combined Blue Light Irradiation/Drug.

Authors
  • Zhuang, Jianjian1
  • Liu, Junsong2
  • Gao, Xuan3
  • Li, Hongdong4
  • 1 State Key Laboratory of Superhard Materials, Jilin University, Changchun 130012, China. [email protected] , (China)
  • 2 State Key Laboratory of Superhard Materials, Jilin University, Changchun 130012, China. [email protected] , (China)
  • 3 State Key Laboratory of Superhard Materials, Jilin University, Changchun 130012, China. [email protected] , (China)
  • 4 State Key Laboratory of Superhard Materials, Jilin University, Changchun 130012, China. [email protected] , (China)
Type
Published Article
Journal
International Journal of Molecular Sciences
Publisher
MDPI AG
Publication Date
May 15, 2018
Volume
19
Issue
5
Identifiers
DOI: 10.3390/ijms19051464
PMID: 29762467
Source
Medline
Keywords
License
Unknown

Abstract

The cell viability and apoptosis of tumor U937 cells treated by blue light (BL) irradiation have been examined. BL irradiation can specially inhibit the proliferation and promote the apoptosis of U937 cells, relating to the production of reactive oxygen species (ROS) and the decline of mitochondrial membrane potential (ΔΨm). The apoptosis is further associated with varying downregulated B-cell lymphoma-extra large (Bcl-XL) and B-cell lymphoma 2 (Bcl-2) genes, upregulated Bcl-2-associated X (Bax) gene, the activation of caspase-3 and caspase-9, and the cleavage of poly (ADP-ribose) polymerase (PARP) by the BL irradiation process. Moreover, BL irradiation induced proliferation inhibition is higher than that treated by a common chemotherapeutic drug of homoharringtonine (HHT). When we synergize BL irradiation with HHT (BL-HHT), a higher proliferation inhibition is obtained than that treated by BL irradiation or HHT alone. These results are helpful for establishing a low toxicity and high efficiency strategy of BL irradiation for clinical treatment of acute myeloid leukemia, not limited to U937 cells.

Report this publication

Statistics

Seen <100 times