Affordable Access

Inhibition of Androgen Receptor-Mediated Transcription by Amino-Terminal Enhancer of split

Authors
  • Xin Yu
  • Peng Li
  • Robert G. Roeder
  • Zhengxin Wang
Publisher
American Society for Microbiology
Publication Date
Jul 01, 2001
Source
PMC
Keywords
Disciplines
  • Biology
License
Unknown

Abstract

A yeast two-hybrid assay has identified an androgen-dependent interaction of androgen receptor (AR) with amino-terminal enhancer of split (AES), a member of the highly conserved Groucho/TLE family of corepressors. Full-length AR, as well as the N-terminal fragment of AR, showed direct interactions with AES in in vitro protein-protein interaction assays. AES specifically inhibited AR-mediated transcription in a well-defined cell-free transcription system and interacted specifically with the basal transcription factor (TFIIE) in HeLa nuclear extract. These observations implicate AES as a selective repressor of ligand-dependent AR-mediated transcription that acts by directly interacting with AR and by targeting the basal transcription machinery.

Report this publication

Statistics

Seen <100 times