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Inhibition of KIF20A by transcription factor IRF6 affects the progression of renal clear cell carcinoma

Authors
  • Ma, Xinwei1, 2
  • Wang, Xiaoqi3
  • Dong, Qian2
  • Pang, Hongquan2
  • Xu, Jianming2
  • Shen, Junkang1
  • Zhu, Jianbing2
  • 1 The Second Affiliated Hospital of Soochow University, No.1055 Sanxiang Road, Suzhou, Jiangsu, 215004, China , Suzhou (China)
  • 2 Suzhou Science and Technology Town Hospital, The Affiliated Suzhou Science and Technology Town Hospital of Nanjing Medical University, No.1 Lijiang Road, high Tech Zone, Suzhou, Jiangsu, 215153, China , Suzhou (China)
  • 3 The First Affiliated Hospital of Anhui University of Science and Technology, Huainan, Anhui, 232001, China , Huainan (China)
Type
Published Article
Journal
Cancer Cell International
Publisher
Springer (Biomed Central Ltd.)
Publication Date
May 03, 2021
Volume
21
Issue
1
Identifiers
DOI: 10.1186/s12935-021-01879-y
Source
Springer Nature
Keywords
License
Green

Abstract

BackgroundRenal clear cell carcinoma (ccRCC) is one of the most common malignant tumors, whose incidence is increasing year by year. IRF6 plays an important role in the occurrence of tumors, although there is yet no report on its expression in ccRCC.MethodsThe expression of IRF6 and KIF20A in ccRCC was predicted by GEPIA and HAP databases. In addition, GEPIA database predicted the relationship between IRF6 and KIF20A expressions and the pathological staging, overall survival, and disease-free survival of ccRCC. The possible binding sites of IRF6 and KIF20A promoters were predicted by JASPAR database and verified by luciferase and ChIP assays. The specific effects of IRF6 on ccRCC cell proliferation, invasion and apoptosis were subsequently examined at both cellular level and animal level.ResultsThe database predicted down-regulated IRF6 expression in renal carcinoma tissues and its correlation with poor prognosis. IRF6 overexpression inhibited cRCC cell proliferation, invasion and migration. In addition, up-regulated KIF20A expression in renal carcinoma tissues and its association with prognosis were also predicted. Interference with KIF20A inhibited the proliferation, invasion, and migration of ccRCC cells. Finally, we confirmed that KIF20A is a functional target of IRF6 and can partially reverse the effects of IRF6 on the proliferation, invasion and migration of ccRCC cells. Conclusion: Inhibition of KIF20A by transcription factor IRF6 affects cell proliferation, invasion and migration in renal clear cell carcinoma.

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