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Inhibition of berberine hydrochloride on Candida albicans biofilm formation.

Authors
  • Huang, Xiaoxue1
  • Zheng, Mingyue2, 3
  • Yi, Yuling1
  • Patel, Anamica4
  • Song, Zhen1
  • Li, Yan5
  • 1 College of Medical Technology, Chengdu University of Traditional Chinese Medicine, Sichuan, 611137, China. , (China)
  • 2 School of Health and Rehabilitation, Chengdu University of Traditional Chinese Medicine, Sichuan, 611137, China. , (China)
  • 3 Adelaide Medical School, University of Adelaide, Adelaide, SA, 5005, Australia. , (Australia)
  • 4 Observatory Evidence Service, Public Health Wales, Cardiff, CF10 4BZ, UK.
  • 5 College of Medical Technology, Chengdu University of Traditional Chinese Medicine, Sichuan, 611137, China. [email protected] , (China)
Type
Published Article
Journal
Biotechnology Letters
Publisher
Springer-Verlag
Publication Date
Jun 15, 2020
Identifiers
DOI: 10.1007/s10529-020-02938-6
PMID: 32557120
Source
Medline
Keywords
Language
English
License
Unknown

Abstract

This study aimed to investigate the inhibitory effect of berberine hydrochloride (BH) on Candida albicans (C.albicans) ATCC10231 biofilm formation. This paper found a positive correlation between the concentration of BH and its inhibitory effect on the cellular activity of early biofilms because we found that 128 and 32 μg/mL BH significantly inhibited biofilm formation (P < 0.05). BH significantly inhibited the cellular activity in early biofilms, destroyed the microscopic morphology of C.albicans and reduced the thickness of the biofilm. Both 128 and 32 μg/mL concentration solutions of BH significantly inhibited biofilm formation (P < 0.05). We found that the inhibitory effect of BH solution was positively correlated with its concentration and 128 μg/mL BH was better than 4 μg/mL fluconazole. Additionally, the results of RT-PCR indicated that 128 and 32 μg/mL BH inhibited the expression of EFG1, HWP1, ECE1, and ALS1 (P < 0.05). The efficacy of BH in inhibiting the formation of C.albicans biofilm by killing the cells in the biofilm and destroying its structure; and the mechanism may be to down-regulate the expression of EFG1, HWP1, ECE1, and ALS1 in hyphae formation, thereby, retarding the morphological transformation of C. albicans.

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