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Inhibition of ASCT2 is essential in all-trans retinoic acid-induced reduction of adipogenesis in 3T3-L1 cells.

Authors
  • Takahashi, Katsuhiko
  • Uchida, Natsumi
  • Kitanaka, Chisato
  • Sagara, Chiaki
  • Imai, Masahiko
  • Takahashi, Noriko
Type
Published Article
Journal
FEBS Open Bio
Publisher
Wiley (John Wiley & Sons)
Publication Date
Jan 01, 2015
Volume
5
Pages
571–578
Identifiers
DOI: 10.1016/j.fob.2015.06.012
PMID: 26236584
Source
Medline
Keywords
License
Unknown

Abstract

Vitamin A has preventive effects on obesity. All-trans retinoic acid (ATRA), the active form of vitamin A, inhibits lipid accumulation in 3T3-L1 cells in an experimental adipogenesis model. We found that ATRA suppressed up-regulation of the amino acid transporter, Asct2, in adipogenerating 3T3-L1 cells. We observed that Asct2 was up-regulated at 1 day after adipogenesis stimuli. The Asct2 inhibitor l-γ-glutamyl-p-nitroanilide (GPNA) decreased lipid accumulation. Glutamine-free conditions also suppressed adipogenesis. Suppression of adipogenesis by ATRA may be through Asct2 reduction. These results indicate that Asct2 could be a target for obesity prevention and treatment.

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