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Inhalation of high-concentration hydrogen gas attenuates cognitive deficits in a rat model of asphyxia induced-cardiac arrest

Authors
  • Huang, Lei1
  • Applegate II, Richard L.2
  • Applegate, Patricia M.3
  • Gong, Lei4
  • Ocak, Umut5
  • Boling, Warren1
  • Zhang, John H.6
  • 1 Department of Neurosurgery, School of Medicine, Loma Linda University, Loma Linda, CA, USA
  • 2 Department of Anesthesiology and Pain Medicine, School of Medicine, University of California, Davis, Sacramento, CA, USA
  • 3 Department of Cardiology, School of Medicine, University of California, Davis, Sacramento, CA, USA
  • 4 Affiliated Hospital to Zunyi Medical University, Zunyi, Guizhou Province, China
  • 5 Department of Basic Sciences, Division of Physiology, School of Medicine, Loma Linda University, Loma Linda, CA, USA
  • 6 Department of Anesthesiology, School of Medicine, Loma Linda University, Loma Linda, CA, USA
Type
Published Article
Journal
Medical Gas Research
Publisher
Wolters Kluwer - Medknow
Publication Date
Sep 23, 2019
Volume
9
Issue
3
Pages
122–126
Identifiers
DOI: 10.4103/2045-9912.266986
PMID: 31552874
PMCID: PMC6779004
Source
PubMed Central
Keywords
License
Green

Abstract

Cognitive deficits are a devastating neurological outcome seen in survivors of cardiac arrest. We previously reported water electrolysis derived 67% hydrogen gas inhalation has some beneficial effects on short-term outcomes in a rat model of global brain hypoxia-ischemia induced by asphyxia cardiac arrest. In the present study, we further investigated its protective effects in long-term spatial learning memory function using the same animal model. Water electrolysis derived 67% hydrogen gas was either administered 1 hour prior to cardiac arrest for 1 hour and at 1-hour post-resuscitation for 1 hour (pre- & post-treatment) or at 1-hour post-resuscitation for 2 hours (post-treatment). T-maze and Morris water maze were used for hippocampal memory function evaluation at 7 and 14 days post-resuscitation, respectively. Neuronal degeneration within hippocampal Cornu Ammonis 1 (CA1) regions was examined by Fluoro-Jade staining ex vivo . Hippocampal deficits were detected at 7 and 18 days post-resuscitation, with increased neuronal degeneration within hippocampal CA1 regions. Both hydrogen gas treatment regimens significantly improved spatial learning function and attenuated neuronal degeneration within hippocampal CA1 regions at 18 days post-resuscitation. Our findings suggest that water electrolysis derived 67% hydrogen gas may be an effective therapeutic approach for improving cognitive outcomes associated with global brain hypoxia-ischemia following cardiac arrest. The study was approved by the Animal Health and Safety Committees of Loma Linda University, USA (approval number: IACUC #8170006) on March 2, 2017.

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