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The influence of sodium trichloroacetate on the tryptophan fluorescence of sarcoplasmic reticulum ATPase

Authors
  • Lüdi, Hans
  • Hasselbach, Wilhelm
Type
Published Article
Journal
Biochimica et Biophysica Acta (BBA) - Biomembranes
Publisher
Elsevier
Publication Date
Jan 01, 1983
Volume
732
Issue
2
Pages
479–482
Identifiers
DOI: 10.1016/0005-2736(83)90066-4
Source
Elsevier
Keywords
License
Unknown

Abstract

The chaotropic anion trichloroacetate quenches the tryptophan fluorescence of the sarcoplasmic reticulum calcium transport ATPase. Half-maximum quenching was observed at 50 mM trichloroacetate. In contrast to native preparations, in trichloroacetate-treated sarcoplasmic reticulum vesicles a decrease of the tryptophan fluorescence is observed on addition of millimolar concentrations of calcium. It is concluded that trichloroacetate renders the tryptophan fluorescence of the ATPase sensitive to the occupancy of its low-affinity sites.

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