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Influence of the Flavonoid, Quercetin on Antioxidant Status, Lipid Peroxidation and Histopathological Changes in Hyperammonemic Rats

Authors
  • Kanimozhi, Sivamani1
  • Bhavani, Pakkiri1
  • Subramanian, Perumal1
  • 1 Annamalai University, Department of Biochemistry and Biotechnology, Faculty of Science, Annamalainagar, Chidambaram, Tamil Nadu, 608 002, India , Annamalainagar, Chidambaram (India)
Type
Published Article
Journal
Indian Journal of Clinical Biochemistry
Publisher
Springer India
Publication Date
Aug 11, 2016
Volume
32
Issue
3
Pages
275–284
Identifiers
DOI: 10.1007/s12291-016-0603-8
Source
Springer Nature
Keywords
License
Yellow

Abstract

We have studied the ability of quercetin (a bioflavonoid) in tackling oxidative stress to alleviate the symptoms during ammonium chloride-induced hyperammonemia. Hyperammonemia was induced by the treatment of ammonium chloride (AC) 100 mg/kg b.w for 56 days. Hyperammonemic rats exhibited reduced urea (in plasma) and increased ammonia (in blood), uric acid (in plasma), creatinine (in serum), oxidative stress markers (thiobarbituric acid reactive substances (TBARS) and hydroperoxides (HP) and decreased levels of antioxidants (enzymatic and non-enzymatic) superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), reduced glutathione (GSH) in plasma and tissues (liver and brain) vitamins E and C (in plasma)). The expression of liver inflammatory markers such as, interleukin 6 (IL-6), inducible nitric oxide synthase (iNOS) and nuclear transcription factor-κB (NF-κB) (by western blotting) were investigated. Histological damages (in liver, brain and kidney) were observed under hyperammonemia and the administration of quercetin (1) normalized the histopathological alterations, (2) reduced the levels of TBARS and HP, (3) elevated the antioxidants (SOD, CAT, GPx, GSH, vitamins E and C), (4) declined the activities of liver marker enzymes (AST, ALT and ALP) and (5) down regulated the expression of IL-6, iNOS and NF-κB. Our results suggest that quercetin might exert defense to AC-induced hyperammonemic rats to tackle (1) oxidative stress and (2) inflammation owing to its antioxidant, anti-inflammatory and cytoprotective effects.

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