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Influence of the bound nucleotide on the molecular dynamics of actin.

Authors
  • Hegyi, G
  • Szilágyi, L
  • Belágyi, J
Type
Published Article
Journal
European journal of biochemistry / FEBS
Publication Date
Aug 01, 1988
Volume
175
Issue
2
Pages
271–274
Identifiers
PMID: 2841132
Source
Medline
License
Unknown

Abstract

Rotational dynamics of actin spin-labelled with maleimide probes at the reactive thiol Cys-374 were studied. Replacement of the bound nucleotide by Br8ATP in G-actin and Br8ADP in F-actin causes significant increase of the rotational correlation time of the spin probe, indicating reduced motion in both G and F-actin. The orientation dependence of the electron paramagnetic resonance spectra in oriented F-actin filaments revealed an altered molecular order of the probe when the nucleotide was a Br-substituted one. The bound nucleotide affects the myosin S1 ATPase activation by actin; both Vmax and K(actin) decreased significantly when the bound nucleotide of actin was Br8ADP.

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