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[Influence of albumin and placement time outside the incubator on viability of isolated cytokine-induced killer cells: a simple method for detection].

Authors
Type
Published Article
Journal
Nan fang yi ke da xue xue bao = Journal of Southern Medical University
Publication Date
Volume
33
Issue
7
Pages
1078–1082
Identifiers
PMID: 23895858
Source
Medline

Abstract

CIK cells from different cancer patients present with different growth pattern of the cells clones. Labeling with anti-CD3-FITC and anti-CD56-PE allows convenient counting of the newly generated CD3(+)CD56(+) CIK cells and FQ-AE labeling can be used for quantitative assessment of cell death. Albumin is not necessary in the medium of CIK cells. Prolonged placement (for over 90 min) of CIK cells outside the incubator should be avoided, and the placement time should be shorten as much as possible.

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