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Infectivity of Sf-rhabdovirus variants in insect and mammalian cell lines.

Authors
  • Maghodia, Ajay B1
  • Jarvis, Donald L2
  • 1 GlycoBac, LLC, Laramie, WY 82072, USA.
  • 2 GlycoBac, LLC, Laramie, WY 82072, USA; Department of Molecular Biology, University of Wyoming, Laramie, WY 82071, USA. Electronic address: [email protected]
Type
Published Article
Journal
Virology
Publisher
Elsevier
Publication Date
Dec 01, 2017
Volume
512
Pages
234–245
Identifiers
DOI: 10.1016/j.virol.2017.09.025
PMID: 29024851
Source
Medline
Keywords
License
Unknown

Abstract

Sf-rhabdovirus was only recently identified as an adventitious agent of Spodoptera frugiperda (Sf) cell lines used as hosts for baculovirus vectors. As such, we still know little about its genetic variation, infectivity, and the potential impact of variation on the Sf-rhabdovirus-host interaction. Here, we characterized Sf-rhabdoviruses from two widely used Sf cell lines to confirm and extend information on Sf-rhabdovirus variation. We then used our novel Sf-rhabdovirus-negative (Sf-RVN) Sf cell line to assess the infectivity of variants with and without a 320bp X/L deletion and found both established productive persistent infections in Sf-RVN cells. We also assessed their infectivity using heterologous insect and mammalian cell lines and found neither established productive persistent infections in these cells. These results are the first to directly demonstrate Sf-rhabdoviruses are infectious for Sf cells, irrespective of the X/L deletion. They also confirm and extend previous results indicating Sf-rhabdoviruses have a narrow host range.

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