Affordable Access

deepdyve-link
Publisher Website

Infective Arthritis: Bacterial 23S rRNA Gene Sequencing as a Supplementary Diagnostic Method.

Authors
  • Moser, Claus1
  • Andresen, Keld
  • Kjerulf, Anne
  • Salamon, Suheil
  • Kemp, Michael
  • Christensen, Jens Jørgen
  • 1 Department of Bacteriology, Mycology, and Parasitology, Statens Serum Institut, Copenhagen, and the Departments of Clinical Microbiology.
Type
Published Article
Journal
The Open Microbiology Journal
Publisher
Bentham Science
Publication Date
2008
Volume
2
Pages
85–88
Identifiers
DOI: 10.2174/1874285800802010085
PMID: 19088916
Source
Medline
Keywords
License
Unknown

Abstract

Consecutively collected synovial fluids were examined for presence of bacterial DNA (a 700-bp fragment of the bacterial 23S rRNA gene) followed by DNA sequencing of amplicons, and by conventional bacteriological methods. One or more microorganisms were identified in 22 of the 227 synovial fluids (9,7%) originating from 17 patients. Sixteen of the patients had clinical signs of arthritis. For 11 patients molecular and conventional bacterial examinations were in agreement. Staphylococcus aureus, Streptococcus dysgalactiae subspecies equisimilis and Streptococcus pneumoniae, were detected in synovial fluids from 6, 2 and 2 patients, respectively. In 3 patients only 23S rRNA analysis was positive; 2 synovial fluids contained S. dysgalactiae subspecies equisimilis and 1 S. pneumoniae). The present study indicates a significant contribution by PCR with subsequent DNA sequencing of the 23S rRNA gene analysis in recognizing and identification of microorganisms from synovial fluids.

Report this publication

Statistics

Seen <100 times