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Infectious Agents Identified by Real-Time PCR, Serology and Bacteriology in Blood and Peritoneal Exudate Samples of Cows Affected by Parietal Fibrinous Peritonitis after Caesarean Section

Authors
  • Djebala, Salem1
  • Evrard, Julien2
  • Gregoire, Fabien2
  • Thiry, Damien
  • Bayrou, Calixte1
  • Moula, Nassim
  • Sartelet, Arnaud1
  • Bossaert, Philippe1
  • 1 (P.B.)
  • 2 (F.G.)
Type
Published Article
Journal
Veterinary Sciences
Publisher
MDPI
Publication Date
Sep 13, 2020
Volume
7
Issue
3
Identifiers
DOI: 10.3390/vetsci7030134
PMID: 32933113
PMCID: PMC7558678
Source
PubMed Central
Keywords
Disciplines
  • Article
License
Green

Abstract

The aim of this study was to identify the pathogens potentially involved in parietal fibrinous peritonitis (PFP). PFP is a complication of laparotomy in cattle, characterized by an accumulation of exudate inside a fibrinous capsule. We have studied 72 cases of PFP in Belgian blue cows, confirmed by a standard diagnostic protocol. Blood was collected to evaluate the presence of antibodies for Mycoplasma bovis (M. bovis) , Coxiella burnetii (C. burnetii) and Bovine Herpesvirus 4 (BoHV4 ) by enzyme-linked immunosorbent assays. Peritoneal exudate was obtained from the PFP cavity to perform bacteriological culture, and to identify the DNA of M . bovis , C . burnetii and BoHV4 using real time polymerase chain reaction (qPCR). Bacteriological culture was positive in most peritoneal samples (59/72); Trueperella pyogenes ( T. pyogenes ) (51/72) and Escherichia coli ( E. coli ) (20/72) were the most frequently identified. For BoHV4 , the majority of cows showed positive serology and qPCR (56/72 and 49/72, respectively). Contrariwise, M. bovis (17/72 and 6/72, respectively) and C. burnetii (15/72 and 6/72, respectively) were less frequently detected ( p < 0.0001). Our study proves that PFP can no longer be qualified as a sterile inflammation. Moreover, we herein describe the first identification of BoHV4 and C. burnetii in cows affected by PFP.

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