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Industrial rape-biotechnology. Subproject 7, task 11: isolation of genes by two methods without enzyme purification for increasing the erucid acid content of rape oil Final report / Industrieraps-Biotechnologie. Teilvorhaben 7, Aufgabe 11: Isolierung von Genen mit zwei Methoden ohne Enzymreinigung zur Steigerung des Erucasaeuregehalts von Rapsoel Abschlussbericht

Authors
  • Frentzen, M.
  • Wolter, F.P.
  • univ., hamburg
Publication Date
Jan 01, 1993
Source
OpenGrey Repository
Keywords
Language
German
License
Unknown

Abstract

For introduction of the unsaturated erucid acid into the sn-2 position of glycerol during the triacylglycerol biosynthesis in developing rape seeds, the 1-acylglycerol-3-phosphate acyltransferase (1-Acyl-GPAT) of rape has to be exchanged by the corresponding seed specific enzyme from limnanthes douglasii. Acyltransferases from rape and limnanthes can be isolated by heterologic complementation and by in situ enzymassay based on the functional expression of the cDNAs in yeast cells. Both methods have been developed and optimized avoiding thus the difficult enzyme purification step. (WEN) / Available from TIB Hannover: DtF QN1(25,40) / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische Informationsbibliothek / SIGLE / Bundesministerium fuer Forschung und Technologie (BMFT), Bonn (Germany) / DE / Germany

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